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Title: Verifying The Accuracy Of Analytical Spectrophotometers
Author: J.C. Travis, M.V. Smith, N.K. Winchester, J.C. Colbert
Source: 1995 Measurement Science Conference
Year Published: 1995
Abstract: A variety of Standard Reference Materials (SRMs) for verifying the absorbance and wavelength accuracy of UV/visible spectrometers has been developed and marketed by NIST over the past two decades. Neutral density glass is certified for transmittance density and transmittance at five wavelengths from 440 nm to 635 nm, at six nominal transmittance levels from 0.01 to 0.5. Thin film metal-on-quartz filters extend convenient solid-filter calibration into the UV at nominal transmittances of 0.1, 0.3 and 0.9. Liquid standards spanning UV/visible wavelengths offer several advantages to the user, but at the expense of more complicated procedures for their use. A solution of holmium oxide in flame-sealed quartz cuvettes is available for calibration of the wavelength axis of spectrophotometers using fourteen sharp spectral bands with certified wavelength. Current research efforts are directed to developing sealed-cuvette liquid absorbance standards, and solid filter standards for wavelength calibration and absorbance verification in near infrared spectrophotometers. NIST provides a detailed description of the accuracy uncertainty of each certified value furnished. The end user confounds this uncertainty with the measurement precision of the tested instrument and with the stated tolerance of the test to determine a confidence interval that should properly describe the experimental data for the tested instrument.

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